Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease strongly associated with metabolic dysfunction, but its pathogenesis remains poorly understood. Investigation of circulating metabolites may help in elucidating underlying mechanisms and identify new biomarkers for NAFLD. In this meta-analysis we will examine the association between plasma metabolites and NAFLD as well as liver enzymes using data from four CHARGE cohorts (Rotterdam Study – RS, Avon Longitudinal Study of Parents and Children – ALSPAC, Study of Latinos – SOL, Insulin Resistance Atherosclerosis Family Study – IRASFS).