1536 SNPs are planned for genotyping in Raine in early 2008 including tagged genes on both cardiovascular and depression pathways. A further literature review will be performed once the project has commenced to ensure, during this rapid discovery and replication phase of genetic research, that all additional validated, common CVD and depression susceptibility SNPs are included. With an anticipated 5% of all genotyped SNPs in Raine being significantly associated with our primary outcomes of interest, approx 80 SNPs will be genotyped in ALSPAC. The Illumina Golden Gate assay requires a minimum of 5micro-l of DNA normalized to 50ng/micro-l in TE.

The phenotypic data required for the research in this proposal include:

• Data have on maternal and paternal social background, lifestyle and habits (including diet and physical activity), and medical history by self-completion.
• Details of the mother's medical condition and medical history, the medical history of her partner, socioeconomic and lifestyle characteristics of both parents and the clinical course of the mother's index pregnancy.
• Blood pressure.
• Carotid IMT measured in the far wall of the left common carotid artery.
• Cholesterol measures
• Measures of cognition, attention, emotions and psychotic-like symptoms
• Measures of socioeconomic position and other potential covariates including smoking, alcohol and drug use