We wish to identify those ALSPAC children who have had 3D facial scans, orthoptic assessments, refraction, clinical examination and for whom we have maternal alcohol intake data. We will stratify alcohol exposure as done previously for this cohort into women who drank no alcohol in pregnancy, or less than 1 drink per week, or more than on drink per week (2). In this earlier ALSPCAC paper, effects on child temperament were identified after exposure to greater than 1 drink per week during pregnancy (approx 16% women in that analysis), suggesting there is enough variation in alcohol exposure within the cohort for useful analyses of outcome. We will perform a variety of statistical analyses seeking to examine associations between alcohol exposure and facial anatomical and ophthalmic measurements. For example, category of first trimester alcohol intake as a predictor or modifier of (a) palpebral aperture width (between medial and lateral canthi), (b) distance between the eyes (between the medial canthi) (c) occurrence, direction and magnitude of strabismus (assessed at age 7) and refractive error (outcome data at age 3 for 2000 and at age 15 for approx 5000) and ocular biometry (available for 2900 at age 15).