B2097 - Analysis of correlation patterns in DNA methylation through the lifecourse - 23/10/2013

B number: 
B2097
Principal applicant name: 
Dr Tom Gaunt (University of Bristol, UK)
Co-applicants: 
Dr Caroline Relton (University of Bristol, UK), Prof George Davey Smith (University of Bristol, UK), Mr Geoff Woodward (University of Bristol, UK), Dr Oliver Lyttleton (University of Bristol, UK), Dr Josine Min (University of Bristol, UK), Dr Oliver Stegle (European Bioinformatics Institute (EMBL), UK), Mr Paolo Casale (European Bioinformatics Institute (EMBL), UK)
Title of project: 
Analysis of correlation patterns in DNA methylation through the lifecourse.
Proposal summary: 

Outline

DNA methylation data has recently been generated from ALSPAC samples at multiple timepoints using the Illumina HM450k DNA methylation array. Genome-wide correlation analysis is now possible to (a) identify potential coregulation and (b) to inform dimension reduction strategies to data analysis. It is also possible to explore the correlation between timepoints to determine which CpG sites in the genome are functionally stable, and which change over time.

Aims

1. Windowed analysis across the genome to generate "methylation correlation maps" at all time-points

2. Genome-wide analysis of correlation between variable loci to identify networks of genes that appear to be coregulated

3. Analysis of correlation of methylation between timepoints at each site in the HM450k to identify stable and labile CpGs.

Date proposal received: 
Wednesday, 23 October, 2013
Date proposal approved: 
Wednesday, 23 October, 2013
Keywords: 
Primary keyword: 
Epigenetics